| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139962 |
| Chromosome: | chromosome 16 |
| Location: | 5852960 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCACAGCTCGGGTGTCAAGGCGGTCAG |
| Internal bar code: | ACTGGTGTTAGAATCTCTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 756 |
| LEAP-Seq percent confirming: | 99.2673 |
| LEAP-Seq n confirming: | 3929 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAGCACGTGGAGAGAAA |
| Suggested primer 2: | AAGTCCTACACCATCCGACG |