| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139963 |
| Chromosome: | chromosome 16 |
| Location: | 2586596 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661588 | OPR102 | OctotricoPeptide Repeat Protein 102 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTGTGGCCGCTGCCTGTTGCACACGCA |
| Internal bar code: | GTTTCGTGCTATTCCTCGTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 147 |
| LEAP-Seq percent confirming: | 96.6151 |
| LEAP-Seq n confirming: | 999 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAGGAGCTGAGCATGGTG |
| Suggested primer 2: | GTGAAAATACAGCGGCCAAT |