| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139966 |
| Chromosome: | chromosome 6 |
| Location: | 835397 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g254450 | (1 of 1) K10295 - F-box protein 9 (FBXO9) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGCTGGTGTGAGGGGCAGGGTGTGGGCG |
| Internal bar code: | TGGGATTTGTTTTCTGCTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1185 |
| LEAP-Seq percent confirming: | 99.6343 |
| LEAP-Seq n confirming: | 10354 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGACTCAACGCTCCTCTC |
| Suggested primer 2: | CTACCCCAACAGCACAAGGT |