Insertion junction: LMJ.RY0402.139968_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.139968
Chromosome:chromosome_9
Location:4491149
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g395769 FAP177 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CTGCTCAGCCCAAGCAGCCCACATCCATTC
Internal bar code:CTCACCGTGACGGGTCGGACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:198
LEAP-Seq percent confirming:98.5451
LEAP-Seq n confirming:2303
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GTGACTGCACTGCACCATCT
Suggested primer 2:GTAGATCTCTGCGGAAACGC