| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139974 |
| Chromosome: | chromosome 2 |
| Location: | 1059890 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080750 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCGATGCTGTGGTTCTGCGCGCAGGGT |
| Internal bar code: | GATCTGAGTGGATTGCGGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 307 |
| LEAP-Seq percent confirming: | 95.0666 |
| LEAP-Seq n confirming: | 1214 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTACATGAAGGTGCCGTG |
| Suggested primer 2: | ACATCCTATCACCCTCGCAG |