| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139987 |
| Chromosome: | chromosome 17 |
| Location: | 3852272 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728150 | YKT6 | Endosomal R-SNARE protein, Yky6-family (R.II); (1 of 1) K08516 - synaptobrevin homolog YKT6 (YKT6) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGTGCACGGCAAGGTATCGGTACATTC |
| Internal bar code: | TATTGTACGTATCATAAGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 983 |
| LEAP-Seq percent confirming: | 99.7644 |
| LEAP-Seq n confirming: | 2964 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGCCTGCTTACCTACAA |
| Suggested primer 2: | ATGCAAACCACTTCACCACA |