| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139992 |
| Chromosome: | chromosome 17 |
| Location: | 3904257 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728350 | (1 of 21) PF02469 - Fasciclin domain (Fasciclin) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCGGACTCGAATGGACCCGTTGACTGC |
| Internal bar code: | CACGCACTAGGGCCAAACTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 781 |
| LEAP-Seq percent confirming: | 99.5022 |
| LEAP-Seq n confirming: | 6996 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGGTCTGCTGATGTGT |
| Suggested primer 2: | ACCTCTTCGCTCTGGACGTA |