| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140008 |
| Chromosome: | chromosome 12 |
| Location: | 8475014 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548050 | CYG32 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGACTGAAGCCCACCAATGGCATGGCC |
| Internal bar code: | GCGTGGGTGCCTCGAACGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 826 |
| LEAP-Seq percent confirming: | 98.59 |
| LEAP-Seq n confirming: | 3566 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCCGTGTTATGAAACCA |
| Suggested primer 2: | CACGATTAGAGGCATCAGCA |