Insertion junction: LMJ.RY0402.140015_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.140015
Chromosome:chromosome_13
Location:2132620
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g577900 antisense 5'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):AGTAGCTCGCGGTGGCGGTTCCGGCGGACG
Internal bar code:TTCTTCTCTGATGTATTTCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:76
LEAP-Seq percent confirming:49.1202
LEAP-Seq n confirming:335
LEAP-Seq n nonconfirming:347
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGCACTGACTGCTACTC
Suggested primer 2:AACGTACAGCGTGTGACTGC