Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.140015 |
Chromosome: | chromosome_13 |
Location: | 2132620 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g577900 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGTAGCTCGCGGTGGCGGTTCCGGCGGACG |
Internal bar code: | TTCTTCTCTGATGTATTTCACT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 76 |
LEAP-Seq percent confirming: | 49.1202 |
LEAP-Seq n confirming: | 335 |
LEAP-Seq n nonconfirming: | 347 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCTGCACTGACTGCTACTC |
Suggested primer 2: | AACGTACAGCGTGTGACTGC |