| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.140019 |
| Chromosome: | chromosome 8 |
| Location: | 1723660 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365950 | (1 of 3) PTHR22753:SF1 - TRANSMEMBRANE PROTEIN 68 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGAGCGTGTAGGTGTGCTGGCAGCCCG |
| Internal bar code: | TGGATCGAGAACATAAGGCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1202 |
| LEAP-Seq percent confirming: | 99.4052 |
| LEAP-Seq n confirming: | 16379 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGCACTACACAGCACCG |
| Suggested primer 2: | TGTTGTGGGTGTACGGACTG |