| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140023 |
| Chromosome: | chromosome 16 |
| Location: | 4478333 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670756 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAGTCATGGGCACGGGATCTTCCTTAG |
| Internal bar code: | TGAAGTAGGCGCGCTTCACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 657 |
| LEAP-Seq percent confirming: | 99.3593 |
| LEAP-Seq n confirming: | 8374 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTTCAACTGCTCTTTCCC |
| Suggested primer 2: | AGGCGCAGTATAGGAAAGCA |