| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.140026 |
| Chromosome: | chromosome 14 |
| Location: | 1238763 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616376 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTTTCCCTGCACGACAAGGGCGGACCT |
| Internal bar code: | CCGCCATGGCAGGTAATGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 630 |
| LEAP-Seq percent confirming: | 99.8531 |
| LEAP-Seq n confirming: | 4079 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGTCCCATGCAAGGTTT |
| Suggested primer 2: | ACGCAAAACTTCTGCATGTG |