| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.140084 |
| Chromosome: | chromosome 4 |
| Location: | 1889228 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217950 | (1 of 1) PTHR24347//PTHR24349//PTHR24349:SF84 - SERINE/THREONINE-PROTEIN KINASE // SERINE/THREONINE-PROTEIN KINASE // CALCIUM-DEPENDENT PROTEIN KINASE 13 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCACACATGGCATCCCACGTGTGCGCTC |
| Internal bar code: | GTCTGTGTGCTAGGAAGGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 98.8292 |
| LEAP-Seq n confirming: | 6162 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCAACGGCATGAAATAA |
| Suggested primer 2: | TAGCATCACCTGTGTGGCAT |