| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140093 |
| Chromosome: | chromosome 12 |
| Location: | 5747274 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533000 | PHR2,PHR1 | (1 of 3) K01669 - deoxyribodipyrimidine photo-lyase [EC:4.1.99.3] (phrB); CPD photolyase, class II | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTTGTGACTTGGCGGGACGGATTCTGTG |
| Internal bar code: | TTATAGTGACGGAACCGAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 996 |
| LEAP-Seq percent confirming: | 99.4984 |
| LEAP-Seq n confirming: | 16663 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGTAGGCGTTTGG |
| Suggested primer 2: | TCCTTCACCAGCTCGTCTTT |