| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140106 |
| Chromosome: | chromosome 17 |
| Location: | 550954 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699700 | Hypothetical protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCCCATACTGAAGAACAGCAGTGCTGG |
| Internal bar code: | AGTCACCGGAAGCTTGCGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 467 |
| LEAP-Seq percent confirming: | 77.0601 |
| LEAP-Seq n confirming: | 1384 |
| LEAP-Seq n nonconfirming: | 412 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGATTAGGTGAGTTCGC |
| Suggested primer 2: | TCCAAACCCGTGCTAATTTC |