| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140106 |
| Chromosome: | chromosome 17 |
| Location: | 551066 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699700 | Hypothetical protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCAACTGAAGATGCGCCAGAGAGGCAA |
| Internal bar code: | GCATAGGTCCAAGCCTCAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 80.9079 |
| LEAP-Seq n confirming: | 6577 |
| LEAP-Seq n nonconfirming: | 1552 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGATTAGGTGAGTTCGC |
| Suggested primer 2: | CCTTGGGGTATTTCATGCAG |