| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.140118 |
| Chromosome: | chromosome 10 |
| Location: | 5441971 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458500 | ASK1,AHD2 | Aspartate kinase; (1 of 1) K00928 - aspartate kinase (lysC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCGGTTGCGGGCTTCGGGCCGCTGGAC |
| Internal bar code: | ACAAGGGGGCGGCCGCGCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 681 |
| LEAP-Seq percent confirming: | 99.8082 |
| LEAP-Seq n confirming: | 10410 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGCCATGTTAGTTGAGC |
| Suggested primer 2: | ACATCCAAGACCGTCACTCC |