| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.140129 |
| Chromosome: | chromosome_5 |
| Location: | 2301139 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g234700 | PYK3 | Pyruvate kinase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTGGAGAAGGTGAGTGGTGTTGGGACAGC |
| Internal bar code: | CGGGGATACTGCGGGCGCGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 872 |
| LEAP-Seq percent confirming: | 98.1424 |
| LEAP-Seq n confirming: | 6657 |
| LEAP-Seq n nonconfirming: | 126 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGCTGGGGTTCTAACTTT |
| Suggested primer 2: | GCCCCAGTATCTACGGAACA |