| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140137 |
| Chromosome: | chromosome 14 |
| Location: | 2750832 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627100 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTAGGCTGTGACGGCGACACAGCTGCA |
| Internal bar code: | GGCAACATCGTGGGAATCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 99.6104 |
| LEAP-Seq n confirming: | 24543 |
| LEAP-Seq n nonconfirming: | 96 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGTAGCAAAGGGTGTGC |
| Suggested primer 2: | GCGGCGTTTGGTCTAGTATC |