| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140147 |
| Chromosome: | chromosome 13 |
| Location: | 840904 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567300 | (1 of 1) PTHR18968//PTHR18968:SF86 - THIAMINE PYROPHOSPHATE ENZYMES // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGAAGTGCAGGACGACAAATATTAACTA |
| Internal bar code: | TCATATGAGTCGTAGAGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 926 |
| LEAP-Seq percent confirming: | 99.3033 |
| LEAP-Seq n confirming: | 6414 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGCTACTGCTACTGTCG |
| Suggested primer 2: | CCTTCTCTGCACCACCATTT |