Insertion junction: LMJ.RY0402.140173_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.140173
Chromosome:chromosome 8
Location:2229914
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g369850 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGCAGGATGAGCCAGTGTGATTGCTAA
Internal bar code:CACGTAAGACTGCATGCGTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:886
LEAP-Seq percent confirming:98.8643
LEAP-Seq n confirming:4788
LEAP-Seq n nonconfirming:55
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGAATGTCATCAGACCGC
Suggested primer 2:GGAGACAAGCAAGTGGAAGC