Insertion junction: LMJ.RY0402.140206_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.140206
Chromosome:chromosome 4
Location:3377197
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g227500 SRR18 Scavenger receptor cysteine rich (SRCR) protein; (1 of 2) IPR001190//IPR006626//IPR011050//IPR017448 - SRCR domain // Parallel beta-helix repeat // Pectin lyase fold/virulence factor // SRCR-like domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCCGCAGGGACGATGACCATGTACTGGG
Internal bar code:GGCACGATGAGACGTACCTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:860
LEAP-Seq percent confirming:99.6735
LEAP-Seq n confirming:3053
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCATCAACACGGAATCCT
Suggested primer 2:ATGCAGGTTTTAAGGCATGG