Insertion junction: LMJ.RY0402.140261_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.140261
Chromosome:chromosome 6
Location:8975199
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g311550 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTACTCAGTCGGCCCCGACGTGCCCGTC
Internal bar code:TCGAAACTCGAGGTTCAGCCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:868
LEAP-Seq percent confirming:99.1815
LEAP-Seq n confirming:15025
LEAP-Seq n nonconfirming:124
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGGACCAGAACACAAGTC
Suggested primer 2:GCGCCATGTAACGACTGTAA