| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140267 |
| Chromosome: | chromosome 2 |
| Location: | 1568659 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084850 | (1 of 4) IPR001810//IPR020683 - F-box domain // Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGTAGAGATGCTGGCAGCCAGAACTGA |
| Internal bar code: | ATTTACGGTTGTTGTTCTCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 247 |
| LEAP-Seq percent confirming: | 63.0807 |
| LEAP-Seq n confirming: | 12994 |
| LEAP-Seq n nonconfirming: | 7605 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACAACCCCTACACTCCGC |
| Suggested primer 2: | TGGCATTTGAGCTCATTCAG |