Insertion junction: LMJ.RY0402.140267_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.140267
Chromosome:chromosome 4
Location:2896189
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g224500 (1 of 1) 3.6.3.17//3.6.3.44 - Monosaccharide-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCCCGTGATGAGGCCCAGCACCGTGAC
Internal bar code:ACCGCTCGAGTCCCGTACCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:792
LEAP-Seq percent confirming:99.2098
LEAP-Seq n confirming:9040
LEAP-Seq n nonconfirming:72
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGGAATAGCAGCAGCAAC
Suggested primer 2:GTGCACGCTTCTGTGTGTTT