| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140279 |
| Chromosome: | chromosome 7 |
| Location: | 3242983 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334150 | (1 of 1) PTHR23097//PTHR23097:SF124 - TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACTCGCTCTCGGTGCAGACCGTAAGGAG |
| Internal bar code: | CCGATGAACGGCTTACTGAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 99.4079 |
| LEAP-Seq n confirming: | 1511 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATATTTAGGGGCCGAGAGC |
| Suggested primer 2: | CGAGTAAGTGCCAGTAGGGC |