Insertion junction: LMJ.RY0402.140286_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.140286
Chromosome:chromosome 13
Location:3734277
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGATCAGGCACGCGCGGACGCGCCTCCTT
Internal bar code:AAGGGGACGCTCTATGCAGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1277
LEAP-Seq percent confirming:99.3994
LEAP-Seq n confirming:6951
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAAGAACTACCGTCGCA
Suggested primer 2:GAGTGGCGCTACAGTGACAA