| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140297 |
| Chromosome: | chromosome 12 |
| Location: | 8772420 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g545500 | (1 of 140) IPR002048 - EF-hand domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACGGAACTATGGCAACGGCACAATTGT |
| Internal bar code: | TTTTCGTTGTATCTGGAATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 420 |
| LEAP-Seq percent confirming: | 99.3038 |
| LEAP-Seq n confirming: | 5135 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCCACTGATGTGGTTGG |
| Suggested primer 2: | GTGTTATTTGGTGGCTCGGT |