| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140338 |
| Chromosome: | chromosome 2 |
| Location: | 1499653 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084150 | (1 of 60) IPR001611 - Leucine-rich repeat | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTCAATCCAGAGCCGGCCCCCTCACCCC |
| Internal bar code: | AATCTGGAACCCCTCTGGTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 914 |
| LEAP-Seq percent confirming: | 99.3966 |
| LEAP-Seq n confirming: | 5436 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGAGGGCGAGTGAAAGAT |
| Suggested primer 2: | GGTGCTTACAACCTTCCCAA |