| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.140345 |
| Chromosome: | chromosome 12 |
| Location: | 148912 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484400 | FKM7 | DNA methylase in the FkbM family; (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCGTGCTTGGAGGCCGAGGTTGAGGTC |
| Internal bar code: | TTCCCCCCGCGGGATTTGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 763 |
| LEAP-Seq percent confirming: | 95.833 |
| LEAP-Seq n confirming: | 36107 |
| LEAP-Seq n nonconfirming: | 1570 |
| LEAP-Seq n unique pos: | 140 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAATACCGCTGCCAATACT |
| Suggested primer 2: | CGGTTTAGTTTGGGCTGGTA |