Insertion junction: LMJ.RY0402.140362_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.140362
Chromosome:chromosome 8
Location:2522123
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCATGTCAAGCCTCAGCGAGCTCCCCGC
Internal bar code:TGGATATATTGTTACTTCGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:556
LEAP-Seq percent confirming:6.48148
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:101
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACACCAGACCAGACCAGACC
Suggested primer 2:AGCGGTCTCCTTCATCTTGA