| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140387 |
| Chromosome: | chromosome 4 |
| Location: | 1190008 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCCGGGCGAGGCCTCCAGCGTGGCCGT |
| Internal bar code: | AATTGGTAAAGAGCGAATTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 828 |
| LEAP-Seq percent confirming: | 99.7702 |
| LEAP-Seq n confirming: | 8683 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTGCTATTGACACTGCC |
| Suggested primer 2: | TCCTGTTTGACAACGAGCAG |