| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.140390 |
| Chromosome: | chromosome 2 |
| Location: | 6352880 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115350 | (1 of 2) 2.7.7.59 - [Protein-PII] uridylyltransferase / Uridylyl removing enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACACCGCATACGTGGGCTTGCCTTAAGC |
| Internal bar code: | CCCCCTAGCCGCCGACTACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 448 |
| LEAP-Seq percent confirming: | 99.3571 |
| LEAP-Seq n confirming: | 27044 |
| LEAP-Seq n nonconfirming: | 175 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTGCTCAACTATTGGCT |
| Suggested primer 2: | TCATGTCTGGCACTCGTAGC |