| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140406 |
| Chromosome: | chromosome 6 |
| Location: | 4932977 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279500 | CPL6 | Conserved in the Plant Lineage; (1 of 1) PTHR15852:SF8 - CHAPERONE PROTEIN DNAJ-LIKE PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCCTGACACGCCGCACTTCGTGCCACA |
| Internal bar code: | GGCAATGGTGTTGGTTTCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1168 |
| LEAP-Seq percent confirming: | 99.6333 |
| LEAP-Seq n confirming: | 26901 |
| LEAP-Seq n nonconfirming: | 99 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCGCGCAGTATATCAAA |
| Suggested primer 2: | GCACCGTACAAGCTACACGA |