| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.140414 |
| Chromosome: | chromosome 11 |
| Location: | 888760 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467654 | (1 of 1) K13117 - ATP-dependent RNA helicase DDX35 [EC:3.6.4.13] (DHX35) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGACCGTGACCGTGAACTTGATCGGCGT |
| Internal bar code: | GGGGAAACCCATCGCCGGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 105 |
| LEAP-Seq percent confirming: | 23.9726 |
| LEAP-Seq n confirming: | 350 |
| LEAP-Seq n nonconfirming: | 1110 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGCCTAATCATCTCATCC |
| Suggested primer 2: | GGCACTCCAAACCTGTGACT |