| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140420 |
| Chromosome: | chromosome 6 |
| Location: | 7493152 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g300350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGGTTTGGCTTCGACTTATGCCGGTGT |
| Internal bar code: | GTGGATCACAGTTATATACCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 712 |
| LEAP-Seq percent confirming: | 97.8508 |
| LEAP-Seq n confirming: | 1548 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGGTTGGGTCCAGTAAGG |
| Suggested primer 2: | GTGGCTCTCAGCTTGTAGGG |