Insertion junction: LMJ.RY0402.140422_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.140422
Chromosome:chromosome 5
Location:763475
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g248550 (1 of 1) K16276 - zinc finger protein-like protein (K16276, BTS) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGAGCTGAATGAGCGTGTGCTAGTCGAC
Internal bar code:TTGTAATAGCGCAGGTAGCCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:664
LEAP-Seq percent confirming:96.4471
LEAP-Seq n confirming:3719
LEAP-Seq n nonconfirming:137
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AACCATGACCCGATCCTGTA
Suggested primer 2:GAATGTGTGTGTTTGCGGAC