| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140425 |
| Chromosome: | chromosome_1 |
| Location: | 1711195 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g009200 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGAGGGAATTGCCCCGCGGTGATTATTGCG |
| Internal bar code: | TCTGCTTCTACGAATGTAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1127 |
| LEAP-Seq percent confirming: | 99.6428 |
| LEAP-Seq n confirming: | 7810 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACTGAACAGACTGGGGC |
| Suggested primer 2: | TGGGAGTTGCAGGAGGATAC |