Insertion junction: LMJ.RY0402.140427_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.140427
Chromosome:chromosome 17
Location:4521469
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g732600 GOX5 Glyoxal oxidase 5; (1 of 1) IPR009880//IPR011043//IPR014756//IPR015202//IPR016187 - Glyoxal oxidase, N-terminal // Galactose oxidase/kelch, beta-propeller // Immunoglobulin E-set // Domain of unknown function DUF1929 // C-type lectin fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAACGCATGGTCACATGAAGACACCAGGG
Internal bar code:TCGAAGCGTCGTCTCGAGGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:375
LEAP-Seq percent confirming:72.3036
LEAP-Seq n confirming:4592
LEAP-Seq n nonconfirming:1759
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGTAGGAAAGCAGTGGTGGA
Suggested primer 2:GGAAACGCTACTTGAGGCTG