| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140438 |
| Chromosome: | chromosome 10 |
| Location: | 2852540 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439750 | (1 of 6) PTHR24114:SF2 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN 74 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTACTTCACACTGCATGCTCACGCGTTT |
| Internal bar code: | ACATTGTGGCGGGGCCTGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 129 |
| LEAP-Seq percent confirming: | 45.4798 |
| LEAP-Seq n confirming: | 1147 |
| LEAP-Seq n nonconfirming: | 1375 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCGATATGGGGTTATGG |
| Suggested primer 2: | CCAACTGAGGTGGGTGTTCT |