| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140449 |
| Chromosome: | chromosome_2 |
| Location: | 2739094 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g093800 | NRX3 | Nucleoredoxin | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGAATCAAGGTTGAAGGCTATTTCACACGA |
| Internal bar code: | GCCGCGTCGCTGTACGTACTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 927 |
| LEAP-Seq percent confirming: | 99.543 |
| LEAP-Seq n confirming: | 13722 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCATCGACAACATCAAC |
| Suggested primer 2: | CTGCTTGATGGTCGTCTCAA |