Insertion junction: LMJ.RY0402.140449_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.140449
Chromosome:chromosome 13
Location:3742502
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTTGTGCCGCCGCTGCCCAGCCCGCCCG
Internal bar code:ATGAGCTTGTCCGCAGATGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:738
LEAP-Seq percent confirming:96.9913
LEAP-Seq n confirming:1225
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GATGGGGAAGTAAGAAGGGG
Suggested primer 2:CGATACCAAGGAGACCCTGA