| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.140456 |
| Chromosome: | chromosome 7 |
| Location: | 3799022 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338451 | FBP1,GLPX1 | Fructose-1%252C6-bisphosphatase Type II; (1 of 1) PTHR30447 - FRUCTOSE-1,6-BISPHOSPHATASE CLASS 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATATACTCGGTCGCAGGCACATGCAAGGC |
| Internal bar code: | TATGCGCGACGGTCCAGTGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 805 |
| LEAP-Seq percent confirming: | 98.9226 |
| LEAP-Seq n confirming: | 50957 |
| LEAP-Seq n nonconfirming: | 555 |
| LEAP-Seq n unique pos: | 144 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCCCACCTCACAAATTC |
| Suggested primer 2: | TCTCGCCGTAAGTGTCTCCT |