| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.140461 |
| Chromosome: | chromosome 3 |
| Location: | 4094632 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172900 | (1 of 2) PF11371 - Protein of unknown function (DUF3172) (DUF3172) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGGCTGGGCTGTGCCGCCCACCGGTCC |
| Internal bar code: | ACGACGCGCATGCTCTGCAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 667 |
| LEAP-Seq percent confirming: | 99.7682 |
| LEAP-Seq n confirming: | 5166 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGTGCCGGGTATAAAGC |
| Suggested primer 2: | TTATCAGCGCAACTCACCTG |