| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140476 |
| Chromosome: | chromosome 10 |
| Location: | 709125 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422500 | (1 of 1) PF09113 - Peptide-N-glycosidase F, C terminal (N-glycanase_C) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACGGTCTGGATCTGCTGACGCTGCTGG |
| Internal bar code: | CTCTGCAAGGCCTGGCGGCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 197 |
| LEAP-Seq percent confirming: | 54.5624 |
| LEAP-Seq n confirming: | 9101 |
| LEAP-Seq n nonconfirming: | 7579 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAACTTAGCGCAACACCA |
| Suggested primer 2: | TACCCGTGTTGTGAAGCGTA |