| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.140482 |
| Chromosome: | chromosome 10 |
| Location: | 2360284 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCAGCTTTGACTTGGGTGATCATGCCA |
| Internal bar code: | GGGGTACCGGCCAGAGCATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 194 |
| LEAP-Seq percent confirming: | 99.3721 |
| LEAP-Seq n confirming: | 14877 |
| LEAP-Seq n nonconfirming: | 94 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGACACCTCCTCCAGATA |
| Suggested primer 2: | TGTGTTTTCGAGTGCAGGAG |