Insertion junction: LMJ.RY0402.140496_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.140496
Chromosome:chromosome 17
Location:3438321
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g724550 DIP13,NA14 Deflagellation Inducible Protein 13; (1 of 1) K16780 - sjoegren syndrome nuclear autoantigen 1 (SSNA1) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTAATCTGCGGTCTGAAGAAGGCTAGTC
Internal bar code:TGGGAATTGTTATGAGAGGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1007
LEAP-Seq percent confirming:99.8613
LEAP-Seq n confirming:3601
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTTCACAGCCTCTCGCTT
Suggested primer 2:GGCAGGAGACATAACACGGT