| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.140496 |
| Chromosome: | chromosome_17 |
| Location: | 3438321 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g724550 | DIP13 | Deflagellation inducible protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGTAATCTGCGGTCTGAAGAAGGCTAGTC |
| Internal bar code: | TGGGAATTGTTATGAGAGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1007 |
| LEAP-Seq percent confirming: | 99.8613 |
| LEAP-Seq n confirming: | 3601 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTCACAGCCTCTCGCTT |
| Suggested primer 2: | GGCAGGAGACATAACACGGT |