Insertion junction: LMJ.RY0402.140497_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.140497
Chromosome:chromosome_6
Location:2166310
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre06.g265950 DHC3 Flagellar inner arm dynein heavy chain, novel minor type dynein sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CGCCGCTGTCGCTGTACGACGTGCACCCCG
Internal bar code:ACAGGGTGTCCGTCATGTAGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:486
LEAP-Seq percent confirming:64.1051
LEAP-Seq n confirming:23174
LEAP-Seq n nonconfirming:12976
LEAP-Seq n unique pos:67

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCTGTTCACCGACTCCTC
Suggested primer 2:ACCCTCCACCAGGAACATCT