Insertion junction: LMJ.RY0402.140501_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.140501
Chromosome:chromosome 11
Location:599917
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g467611 (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATACGGTGACGGGTTCGGGGAACCGCTCC
Internal bar code:AGCTACTTGACTATAGTGTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:155
LEAP-Seq percent confirming:42.0158
LEAP-Seq n confirming:2397
LEAP-Seq n nonconfirming:3308
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGACAAAGGTTGAAGGTA
Suggested primer 2:GTCGGTCTGGTTGAGTTGGT