| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140520 |
| Chromosome: | chromosome 6 |
| Location: | 5329867 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283250 | POB9 | Proteome of basal body 9 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGAGTGGCAGTTCGCGGATTTGCAGGAA |
| Internal bar code: | CTCATCTACATCGTGTCATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 230 |
| LEAP-Seq percent confirming: | 52.0866 |
| LEAP-Seq n confirming: | 4019 |
| LEAP-Seq n nonconfirming: | 3697 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTATAGACTCCTCCACGC |
| Suggested primer 2: | GAACTGAAGGCAACAGGCTC |